(1) 백혈병 유발 돌연변이 유전자에 특이적으로 결합하여 유전자를 절단함으로써 백혈병 세포를 사멸시키는 DNA분자가위 (DNAzyme) 개발.

Cleavage of BCR–ABL transcripts at the T315I point mutation by DNAzyme promotes apoptotic cell death in imatinib-resistant BCR–ABL leukemic cells. (2013) Leukemia 27, 1650-1658.

Site-specific cleavage of mutant ABL mRNA by DNAzyme is facilitated by peptide nucleic acid binding to RNA substrate. (2012) FEBS Lett. 586, 3865-3869.

Design and kinetic analysis of hammerhead ribozyme and DNAzyme that specifically cleave TEL-AML1 chimeric mRNA. (2008) Biochem. Biophys. Res. Commun. 374, 169-174.

  
 

 

 

(2) 대장암 세포 사멸을 위한 Wnt/beta-catenin 신호전달체계에 작용하는 DNAzyme 개발 (공동연구: 국민대 발효융합학과 오상택 교수)

Oligodeoxyribozymes that cleave beta-catenin messenger RNA inhibit growth of colon cancer cells via reduction of beta-catenin response transcription. (2010) Mol Cancer Ther. 9(6):1894-1902.

 

 

(3) C형 간염 바이러스 유전자 특이적으로 작용하는 항바이러스 DNAzyme, anti-miRNA 펩티드핵산 (PNA) 및 유전자 전달체 개발 (공동연구: 서울대 화학과 민달희 교수)

Suppression of hepatitis C virus genome replication in cells with RNA-cleaving DNAzyme and short hairpin RNA. (2010) Oligonucleotides 20(6):285-296.

Functional delivery of DNAzyme with iron oxide nanoparticles for hepatitis C virus gene. (2012) Biomaterials. 33, 2754-2761

Enhanced tumor-targeted gene delivery by immunolipoplexes conjugated with the humanized anti-TAG-72-Fab' fragments. (2012) Bull. Korean Chem. Soc. 33 (2), 651-656.

Antiviral Efficacy of a Short PNA Targeting microRNA-122 Using Galactosylated Cationic Liposome as a Carrier for the Delivery of the PNA-DNA Hybrid to Hepatocytes. (2013) Bull. Korean Chem. Soc. 34, 735-742.

  
 

 

 

(4) siRNA에 의한 세포 내 유전자 발현 억제 분자 기전 연구 (공동연구; 경희대 화학과 하상수 교수)

Sniffing for Gene-Silencing Efficiency of siRNAs in HeLa Cells in Comparison with That in HEK293T Cells: Correlation Between Knockdown Efficiency and Sustainability of siRNAs Revealed by FRET-Based Probing. (2013) Nucleic Acid Ther. 23, 152-159.

FRET-based probing to gain direct information on siRNA sustainability in live cells: asymmetric degradation of siRNA strands. (2011) Mol. Biosyst. 7(7):2110-2113.